Techniques of assisted reproduction
Artificial examination by husband (AIH)
Artificial insemination by donor (AID)
- Cases of severe male factors
- Single women
- Women with female partners
In vitro fertilization (IVF)
1 / Ovarian hormonal stimulationThe first step is to start a hormonal treatment to induce a hyperstimulation of the ovaries which is monitored with ultrasound scan and hormone tests. When the stimulation of the ovaries is optimal a follicular puncture is performed. This first process lasts for 10 to 12 days.
2 / Follicular punctureIt is a painless process as it is performed under sedation in an operating theatre. It takes about 15 minutes. From this puncture the gynaecologist obtains the follicular liquid in which the embryologist identifies the eggs. On the same day of the puncture, a semen sample from the husband is obtained to inseminate the eggs
3 / InseminationIt is a process by which the oocytes are put in contact with the spermatozoa. This process can be performed in two different ways depending on the case. In vitro fertilization (IVF) The oocytes (eggs) obtained from the puncture are put together with an appropriate amount of spermatozoa so that the normal course of fecundation takes place.
Intracitoplasmatic sperm injection (ICSI)With the help of a micromanipulator a single spermatozoon (sperm) is injected into an ovum (egg). The spermatozoa are obtained either through ejaculation or from a testicular biopsy in case of absence of spermatozoa in the semen.
4 / Embryo cultureOn the day after the insemination the fertilised eggs are identified. They are now embryos. These will be cultivated in an in vitro culture medium in the laboratory so that they start to develop (embrionary division).
5 / Embryo transferOn the second or third day after the follicular puncture the embryo transfer is performed. In this process, 1 or 2 embryos are placed in the patient’s uterus through the vagina with the help of an ultrasound scan. It is a short painless process which does not require any sort of anaesthetics. After the embryo transfer, the patient will have to remain in the clinic, resting for about 20 minutes, before she will be allowed to go home. It is recommendable to lead a restful life until the pregnancy test that will take place 12 days after the transfer.
6 / Embryo CryopreservationThe remaining embryos that are not placed in the patient’s uterus on the day of the embryo transfer will be frozen. These can be used later in case the pregnancy is not achieved in the first cycle or for a later second pregnancy. The embryos will be stored in the embryo bank of our laboratory at GIREXX.
- Interview with the gynaecologist.
- Assessment of the recipient’s personal and family medical history.
- Exploració ginecològica completa.
- Complete gynaecological examination.
- Analysis of medical tests. A Patient’s Restricted Area is available to GIREXX patients through which you can send to us all the required documents in a totally confidential manner.
- Detailed explanation of the donation process and the tests performed on donors.
- Information about the treatment (synchronisation with the donor).
- Seminogram and semen cryoconservation.
- Embryo transfer: In this process, 1 or 2 embryos are placed in the patient’s uterus through the vagina with the help of an ultrasound scan. It is a short painless process which does not require any sort of anaesthetics.
Frozen embryo transfer
Donated embryo reception
Preimplantation genetic diagnosis(PGD)
Who can benefit from a PGD?
- Patients with genetic disorders and who want to prevent its transmission to the foetus.
- Carriers of translocations, inversions and other chromosomal disorders.
- P Infertile patients who have had miscarriages repetitively.
- Patients with implantation failures in previous IVF cycles.
- Patients over 35 years of age who have already undergone IVF treatments with more than 3 embryo transfers and with repetitive implantation failures.
- Carriers of oncogenes (BRCA I, II, etc…) who want to prevent their transmission to the foetus.
How is a PGD performed?After an IVF cycle, a PGD is carried out by performing an embryo biopsy when it has a size of 7 or 8 cells. The extracted cell is studied to determine whether it is affected by the suspected genetic abnormality. After this process, only healthy embryos will be transferred into the patient’s uterus.